Correlation between Single nucleotide polymorphism (rs20541 A>G) and Serum Interleukin -13 In Asthma

Abstract

Asthma is a complex disease with factors owing to heredity and exposures that are environmental resulting in chronic airway inflammation. Differences in immune related genes may lead to susceptibility to disease and alterations of cytokines. The present case–control study aimed to assess the relationship of IL13 single nucleotide polymorphism (SNP) rs20541 (A>G) to asthma risk, and its role in serum IL-13 levels according to various genotypes. A total of 118 subjects were recruited for the study: 60 with asthma and 58 healthy controls. The genotyping of the IL13 rs20541 (A>G) polymorphism was conducted by Sanger sequencing, and serum IL-13 levels were determined by enzyme-linked immunosorbent assay (ELISA). The genotype and allele frequencies of IL13 rs20541 were not significantly associated with asthma susceptibility (P = 0.120). G allele frequency was 33.33% for asthma patients and 24.14% for the control group, respectively, but this difference did not reach statistical significance (P=0.28). Furthermore, serum IL-13 levels did not significantly vary between all of the genotypes (P = 1.543). The results of the present study demonstrate that the IL13 rs20541 polymorphism is not a risk factor for asthma, or controls serum levels of IL-13, in this population from Iraq. Additional large-scale studies are necessary to validate these findings for GWAS and extensive genetic markers associated with personalized asthma treatments.